anti - ca v 2.1 alpha-1a rabbit polyclonal  (Synaptic Systems)

Journal: Cerebellum (London, England)

Article Title: Purkinje Cell-Specific Ablation of Ca V 2.1 Channels is Sufficient to Cause Cerebellar Ataxia in Mice

doi: 10.1007/s12311-011-0302-1

Figure Lengend Snippet: Generation of conditional Cacna1a Purkinje cell-specific knockout mice. a Schematic representation of the genomic structure of the relevant part of the floxed and recombined Cacna1a alleles. Black boxes indicate exons ( E ). Triangles indicate the position of loxP sites. Arrows indicate the position of the primers used for the RT-PCR. b RT-PCR of cerebellar RNA amplifies a 580-bp product from the non-recombined Cacna1a transcript and a 490-bp product from transcripts that lack exon 4. c – f Anti-Ca V 2.1-α 1A immunostaining in cerebellar sections of P30 WT ( c , e ) and PCα1KO ( d , f ) mice. CN cerebellar nuclei, G granule cell layer, M molecular layer, PC Purkinje cell layer, WM white matter. Roman numbers indicate the cerebellar lobules

Article Snippet: Sections were incubated in 10% heat-inactivated normal horse serum (NHS) with 0.5% Triton-X100 in TBS, for 2 h and then incubated either with primary rabbit polyclonal anti-Ca V 2.1-α 1A antibody (ACC-001, 1:100; Alomone Labs, Jerusalem, Israel), or with rabbit polyclonal anti-Calbindin antibody (CB38, 1:10,000; SWANT, Bellinzona, Switzerland) diluted in 1% NHS with 2% heat-inactivated fetal calf serum and 0.4% Triton-X100 in TBS at 4°C.

Techniques: Knock-Out, Reverse Transcription Polymerase Chain Reaction, Immunostaining

Journal: Cell Reports

Article Title: PRRT2 modulates presynaptic Ca 2+ influx by interacting with P/Q-type channels

doi: 10.1016/j.celrep.2021.109248

Figure Lengend Snippet:

Article Snippet: Anti - Ca v 2.1 alpha-1A Rabbit Polyclonal , Synaptic Systems , Cat. #152203; RRID: AB_2619841.

Techniques: Recombinant, Saline, Protease Inhibitor, BIA-KA, Bradford Assay, Magnetic Beads, Cell Culture, Software